Experimental design for optimization of bioactive compound extraction from peanut kernels

Abstract

To reveal the accurate bioactivity of natural products, extraction is a critical step. The aim of this study was to optimize the extraction conditions by different extraction methods solid-liquid extraction (SLE), ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE) using experimental designs approach. The interested variables were screened for signification by two levels full factorial design. Then, the significant factors were optimized by three levels center-faced central composite design. The optimized conditions were obtained at maximum responses of antioxidant capacities. The responses at optimized conditions were repeatedly determined to verify the validity of calculated models, and it was compared between extraction methods and evaluated the correlation between responses by Pearson’s analysis. The results found that all generated models were significant and reliable (p

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